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In this study we examined the cytotoxicity of dimethyl sulfoxide (DMSO) for the oocyte to which it is added. The oocytes are cryopreserved by the addition of DMSO alone or mixed with extenders. The modified slow freezing method and the DMSO-pickup method were used to cryopreserve unfertilized eggs and in vitro fertilized eggs. When DMSO and cryoprotectant was added together, the survival rate was the same as that of the control with no DMSO. When DMSO was added to the LN2 step of the slow freezing method, the survival rate of unfertilized eggs was improved and that of fertilized eggs was significantly higher than that of the control. These results suggest that DMSO is useful for the cryopreservation of unfertilized oocytes.Expression of Aurora kinase B mRNA in the parafollicular cells of the human thyroid.
The Aurora kinase B (AURKB) gene has been shown to be amplified in a variety of human cancers; however, its precise expression pattern in human thyroid tumors has not been reported. We have detected AURKB transcript in thyroid tissue and analyzed its localization. Human thyroid tissues were obtained at the time of surgical resection for thyroid carcinoma. Total RNA was extracted from paired normal and tumor tissues,
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